Nano-encapsulated tanshinone IIA in PLGA-PEG-COOH inhibits apoptosis and inflammation in cerebral ischemia/reperfusion injury

Abstract Tanshinone IIA has a potential therapeutic effect on cerebral ischemia/reperfusion injury (CIRI). In this study, tanshinone was encapsulated in poly(lactic- co -glycolic acid)-block-poly (ethylene glycol)-carboxylic acid (PLGA-PEG-COOH) nanoparticles, and its efficacy CIRI investigated. Morphology dynamic light scattering analyses were performed to identify optimize nano-formulations. A drug release test conducted using the dialysis method. The cytotoxic of human neuroblastoma cells (SH-SY5Y) brain endothelial capillary (hCMEC/D3) measured MTT assay. protective PLGA-PEG-COOH-encapsulated against evaluated oxygen glucose deprivation/reoxygenation-induced SH-SY5Y/IR middle artery occlusion (MCAO) rats. Results showed that promoted viability inhibited apoptosis ( P < 0.01). Moreover, facilitated invasion repressed inflammation MCAO rats Noteworthy, combined with angiopep-2 peptide presented better inhibitory than alone Angiopep-2 contributes traversing blood–brain barrier by recognizing lipoprotein-related protein expressed cells. conclusion, plus holds promising toward CIRI.